A Portable Lab At Your Fingertips
DxHub is a compact, field-ready molecular testing system ideal for bringing isothermal assays to where they are needed most.
Prepare your assay tube, insert it into a test bay, and let DxHub do the rest.
Flexible Testing Capabilities
-Compatible with DxTests and any other isothermal assays
-Asynchronous processing of up to 8 tests using random-access test bays
Intuitive Plug-and-Play Operations:
-Standalone operation with no end-user calibration or routine maintenance needed
-Fully automated reaction control and signal analysis eliminates the need for laboratory experience or specialized training
Built for Even the Most Challenging Settings:
-Full operation with mains electricity or external battery power
-Ruggedized to withstand rough transport and use in harsh environments
Easy Input and Output:
-Serial interface for barcode scanner and printer
-Secure data transfer over LAN or WiFi, compatible with Laboratory Information Systems (LIS)
DxTests: Rapid, Accurate, Affordable
Explore our Point-of-Care infectious disease tests built for use with DxHub
Case Study: How Researchers at the Broad Institute of MIT and Harvard Used DxHub to Field-Evaluate a CRISPR-Based Mpox Assay During the 2025 Sierra Leone Outbreak
Mpox SHINE, a CRISPR-Cas13 assay developed by the Sabeti Lab, was deployed to Sierra Leone on DxHub for in-country evaluation across 72 clinical specimens — achieving 100% concordance with the qPCR reference standard.
Title
Rapid development and field evaluation of a portable CRISPR-based assay for Mpox during the 2025 Sierra Leone outbreak
Abstract
In early 2025, Sierra Leone experienced a large outbreak of mpox clade IIb, underscoring the urgent need for portable, low-cost diagnostics in decentralized settings. We rapidly developed and field-deployed Mpox SHINE, a CRISPR–Cas13 assay that integrates lyophilized reagents, ambient-temperature lysis, and automated fluorescence detection on a portable device, the DxHub. The assay achieved analytical sensitivity down to 10 copies/µL with minimal hands-on time. In-country evaluation of 56 clinical specimens showed complete concordance with qPCR, with 100% sensitivity (45/45) and 100% specificity (11/11) at the sample level. Mpox SHINE also detected the virus directly from unextracted lesion swabs, maintaining 100% sensitivity and specificity in 16 samples (8 positive, 8 negative). Across extracted and unextracted samples, the mean time-to-result was ∼35 minutes, with all positives detected within 45 minutes. Thus, Mpox SHINE performed on the DxHub demonstrates how CRISPR-based pathogen detection can be rapidly translated into portable tools for the front lines of outbreak response.